Contents

📒 Sazanov 1994

Proton-translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria1

Sciwheel.

Introduction

  • H+-transhydrogenase (H’-Thase) in the IMM https://user-images.githubusercontent.com/40054455/86718515-bd5d8f80-c055-11ea-9f0b-15bae8d8d983.png

  • Animal mitochondria have two matrix-located iso-citrate dehydrogenases, one NAD-linked and the other NADP-linked https://user-images.githubusercontent.com/40054455/86718522-be8ebc80-c055-11ea-98e5-9f68dea80b77.png

  • Transhydrogenase activity from NADPH to NAD + in coupled rat liver mitochondria was less than 10% of the rate of respiration

A Δp-driven substrate cycle between IC and KG mediated by transhydrogenase

https://user-images.githubusercontent.com/40054455/86718526-bfbfe980-c055-11ea-91ea-a718d83b55f0.png https://user-images.githubusercontent.com/40054455/86718524-be8ebc80-c055-11ea-9ddf-100371a3e5d1.png https://user-images.githubusercontent.com/40054455/86718527-c0588000-c055-11ea-9454-c364db06add3.png

Reference


  1. Sazanov LA, Jackson JB. Proton-translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria. FEBS Lett. 1994;344(2-3):109-116. doi:10.1016/0014-5793(94)00370-X. DOI↩︎