📒 Imperial 2014
Enzyme kinetic equations of irreversible and reversible reactions in metabolism1
- Haldane relationship, an equation which can only be used for reversible reactions, connects biochemical thermodynamics and biochemical kinetics (Keq =(Vf KmP) / (Vr KmA))
Kinetic Equations of Reversible Reactions
- Simplest: Michaelis-Menten mechanism.
- Adair irreversible equation is presented only for an enzyme with two active centers.
- both irreversible equations can be simplified to a Michaelis-Menten uni-uni equation by considering saturated the concentration of one of the substrates
- Haldane relationship from these equations can be solved by considering that equilibrium concentrations would led to v = 0
King and Altman (KA) method: http://biokin.com/king-altman/index.html