Contents

📒 Imperial 2014

Enzyme kinetic equations of irreversible and reversible reactions in metabolism1

Sciwheel

Introduction

  • Haldane relationship, an equation which can only be used for reversible reactions, connects biochemical thermodynamics and biochemical kinetics (Keq =(Vf KmP) / (Vr KmA))

Kinetic Equations of Reversible Reactions

Uni-Uni Mechanisms

  • Simplest: Michaelis-Menten mechanism.

https://user-images.githubusercontent.com/40054455/86619038-78514300-bfec-11ea-8113-5a8f5b56570f.png

  • Adair irreversible equation is presented only for an enzyme with two active centers.

Bi-Bi Mechanisms

  • both irreversible equations can be simplified to a Michaelis-Menten uni-uni equation by considering saturated the concentration of one of the substrates
  • Haldane relationship from these equations can be solved by considering that equilibrium concentrations would led to v = 0

https://user-images.githubusercontent.com/40054455/86619041-7a1b0680-bfec-11ea-910b-bc6fa13c713e.png

Multisubstrate Mechanisms

King and Altman (KA) method: http://biokin.com/king-altman/index.html

Reference


  1. Imperial S, Centelles JJ. Enzyme kinetic equations of irreversible and reversible reactions in metabolism. JBM 2014;02(04):24-29. doi:10.4236/jbm.2014.24005. DOI ↩︎