# ðŸ“’ Imperial 2014

Contents

Enzyme kinetic equations of irreversible and reversible reactions in metabolism

^{1}

## Introduction

- Haldane relationship, an equation which can only be used for reversible reactions, connects biochemical thermodynamics and biochemical kinetics (Keq =(Vf KmP) / (Vr KmA))

## Kinetic Equations of Reversible Reactions

### Uni-Uni Mechanisms

- Simplest: Michaelis-Menten mechanism.

- Adair irreversible equation is presented only for an enzyme with two active centers.

### Bi-Bi Mechanisms

- both irreversible equations can be simplified to a Michaelis-Menten uni-uni equation by considering saturated the concentration of one of the substrates
- Haldane relationship from these equations can be solved by considering that equilibrium concentrations would led to v = 0

### Multisubstrate Mechanisms

King and Altman (KA) method: http://biokin.com/king-altman/index.html