Contents

📒 Gregg 2016

Pancreatic β-Cells From Mice Offset Age-Associated Mitochondrial Deficiency With Reduced KATP Channel Activity 1

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Introduction

Aging is accompanied by impaired glucose homeostasis and an increased risk of type 2 diabetes, culminating in the failure of insulin secretion from pancreatic β-cells.

Methods

Beta-cells were harvested from human and mouse.

NAD(P)H fluorescence lifetime imaging (FLIM) to image islet metabolism.

β-Cell membrane potential and KATP conductance were measured.

Time-lapse Imaging of NAD(P)H, ATP/ADP, and Ca2+.

Results and discussion

KATP channel and calcium oscillations:

https://user-images.githubusercontent.com/40054455/103525678-9bd14980-4eba-11eb-80d2-3710f527e3c3.png

Conclusion

  • Aged human and mouse β-cells exhibit similar defects in mitochondrial glucose sensing
  • But mouse ones showed adaptation by decreasing the conductance of KATP channels.

  1. Gregg T, Poudel C, Schmidt BA, Dhillon RS, Sdao SM, Truchan NA, et al. Pancreatic β-Cells From Mice Offset Age-Associated Mitochondrial Deficiency With Reduced KATP Channel Activity. Diabetes. 2016 Jun 9;65(9):2700–10. PMC5001174 ↩︎