📒 Gregg 2016
Pancreatic β-Cells From Mice Offset Age-Associated Mitochondrial Deficiency With Reduced KATP Channel Activity 1
Aging is accompanied by impaired glucose homeostasis and an increased risk of type 2 diabetes, culminating in the failure of insulin secretion from pancreatic β-cells.
Beta-cells were harvested from human and mouse.
NAD(P)H fluorescence lifetime imaging (FLIM) to image islet metabolism.
β-Cell membrane potential and KATP conductance were measured.
Time-lapse Imaging of NAD(P)H, ATP/ADP, and Ca2+.
Results and discussion
KATP channel and calcium oscillations:
- Aged human and mouse β-cells exhibit similar defects in mitochondrial glucose sensing
- But mouse ones showed adaptation by decreasing the conductance of KATP channels.